Coding

Part:BBa_K4724006:Design

Designed by: Qingqing zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)


IsPETaseS93_I94insE-6xHis Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 549
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to purify the IsPETase protein, so we attached the 6xHis tag after IsPETase.

Source

It's a new part, which is a mutant of IsPETase from Ideonella sakaiensis.

References